Hitrap nhs-activated hp manual

HiTrap NHS-activated HP characteristics. Column volumes 1 ml and 5 ml Column dimensions (i.d. × h) × cm (1 ml) and × cm (5 ml) Degree of substitution 10 μmol NHS groups/ml medium Spacer arm atom Bead structure Highly cross-linked spherical agarose, 6% Mean particle size 34 μm. HiTrap adapters and instruction manual included. Application HiTrap ® NHS-Activated Cartridges are used in affinity chromatography, protein chromatography and activated or functionalized matrices. AS 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Median particle size of the cumulative volume distribution. 2 Dynamic binding capacity measured at 1 mL/min (1 min residence time) using M sodium acetate, pH 3 Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of cm/h in a PEEK /50 .

HiTrap™ NHS-activated HP 1 ml and 5 ml HiTrap NHS-activated HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the medium, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid. Why is the recommended coupling time for HiTrap NHS-Activated HP only minutes whereas the time for coupling on NHS-Activated 4 Fast Flow is h according to the different instruction manuals?. HiTrap® NHS activated HP columns are prepacked with NHS activated Sepharose® High Performance. This high-performance medium is designed for easy coupling of ligands containing primary amines. HiTrap® NHS-activated HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with.

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